Composite

Part:BBa_K4409015:Design

Designed by: Kexin Fei   Group: iGEM22_Worldshaper-HZ   (2022-09-20)


gRNA (AAG+ BBa_K3859000)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We utilized the BBa_K2961003 sequence designed by previous groups to construct the Cas12 handle and BBa_K3859000 designed by GreatBay_SZ to construct the guide sequence.


Source

The sequence we designed is based on BBa_K2961003 for Cas12 handle with AAG present. Also, BBa_K3859000 is utilized to design the guide sequence.

References